Phosphotyrosine Antibodies for Flow Cytometry

105 matching Phosphotyrosine antibodies for Flow Cytometry after applying filters

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Supplier data
BioLegend 309310 Phosphotyrosine antibody, 309310, BioLegend. Human T leukemia cell line Jurkat was cultured in serum-free media overnight and treated with 5mM H2O2 (filled histogram) or PBS (open histogram) for 15 minutes. The cells were then fixed with 2% paraformaldehyde, permeabilized with 90% methanol and stained with anti-phosphotyrosine (clone PY20) PE.

Phosphotyrosine antibody, 309310, BioLegend. Human T leukemia cell line Jurkat was cultured in serum-free media overnight and treated with 5mM H<sub>2</sub>O<sub>2</sub> (filled histogram) or PBS (open histogram) for 15 minutes. The cells were then fixed with 2% paraformaldehyde, permeabilized with 90% methanol and stained with anti-phosphotyrosine (clone PY20) PE.

Mouse Mono [PY20], 100 ug
Mam
FACS

Supplier data
BioLegend 309304

Phosphotyrosine antibody, 309304, BioLegend. Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-phosphotyrosine antibody (clone PY-20). <br>Lane 1, serum-starved Hela cells; Lane 2, serum-starved Hela cells following serum addition for 4 hrs.<br> Lane 2 shows an upregulation of tyrosine phosphorylated proteins after serum addition. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.

Mouse Mono [PY20], 100 ug
Bac, Bv, Cel, Ch, Cn, Dk, Dr, Eq, Eu, Fe, Fi, Fr, Gf, GP, Gt, Ha, Hu, Mam, Mo, Pl, Po, Pr, Pro, Rb, Rt, Sh, Vi, Xe, Yt, Zf
FACS, IF, IP, WB

IF, IP, WB

Supplier data
BioLegend 309312 Phosphotyrosine antibody, 309312, BioLegend. Human T leukemia cell line Jurkat was cultured in serum-free media overnight and treated with 5mM H2O2 (filled histogram) or PBS (open histogram) for 15 minutes. The cells were then fixed with 2% paraformaldehyde, permeabilized with 90% methanol and stained with anti-phosphotyrosine (clone PY20) APC.

Phosphotyrosine antibody, 309312, BioLegend. Human T leukemia cell line Jurkat was cultured in serum-free media overnight and treated with 5mM H<sub>2</sub>O<sub>2</sub> (filled histogram) or PBS (open histogram) for 15 minutes. The cells were then fixed with 2% paraformaldehyde, permeabilized with 90% methanol and stained with anti-phosphotyrosine (clone PY20) APC.

Mouse Mono [PY20], 100 ug
Bac, Mam
FACS

Supplier data
Invitrogen Antibodies 46-5001-41

Phosphotyrosine antibody, 46-5001-41, Invitrogen Antibodies. Intracellular staining of mouse lymph node cells left untreated (orange histogram) or treated with hydrogen peroxide-activated sodium pervanadate (purple histogram) and stained with Anti-Human/Mouse phospho-Tyrosine PerCP-eFluor® 710 (purple histogram) using the IC Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the lymphocyte gate were used for analysis.

Mouse Mono [pY20], 25 Test
Hu, Mo
FACS

Supplier data
Invitrogen Antibodies 50-5001-41

Phosphotyrosine antibody, 50-5001-41, Invitrogen Antibodies. Intracellular staining of normal human peripheral blood cells left untreated (orange histogram) or treated with hydrogen-peroxide-activated sodium pervanadate (purple histogram) and stained with 0.06 µg of Anti-Human/Mouse phospho-Tyrosine eFluor® 660 (purple histogram) using the IC Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Cells in the lymphocyte gate were used for analysis.

Mouse Mono [pY20], 25 Test
Hu, Mo
FACS

Supplier data
Invitrogen Antibodies 46-5001-42

Phosphotyrosine antibody, 46-5001-42, Invitrogen Antibodies. Intracellular staining of mouse lymph node cells left untreated (orange histogram) or treated with hydrogen peroxide-activated sodium pervanadate (purple histogram) and stained with Anti-Human/Mouse phospho-Tyrosine PerCP-eFluor® 710 (purple histogram) using the IC Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the lymphocyte gate were used for analysis.

Mouse Mono [pY20], 100 Test
Hu, Mo
FACS

Supplier data
Invitrogen Antibodies 48-5001-41

Phosphotyrosine antibody, 48-5001-41, Invitrogen Antibodies. Intracellular staining of normal human peripheral blood cells left untreated (orange histogram) or treated with hydrogen-peroxide-activated sodium pervanadate (purple histogram) and stained with 0.06 µg of Anti-Human/Mouse phospho-Tyrosine eFluor® 450 (purple histogram) using the IC Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Cells in the lymphocyte gate were used for analysis.

Mouse Mono [pY20], 25 Test
Hu, Mo
FACS

Supplier data
Invitrogen Antibodies 50-5001-42

Phosphotyrosine antibody, 50-5001-42, Invitrogen Antibodies. Intracellular staining of normal human peripheral blood cells left untreated (orange histogram) or treated with hydrogen-peroxide-activated sodium pervanadate (purple histogram) and stained with 0.06 µg of Anti-Human/Mouse phospho-Tyrosine eFluor® 660 (purple histogram) using the IC Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Cells in the lymphocyte gate were used for analysis.

Mouse Mono [pY20], 100 Test
Hu, Mo
FACS

Supplier data
Invitrogen Antibodies 48-5001-42

Phosphotyrosine antibody, 48-5001-42, Invitrogen Antibodies. Intracellular staining of normal human peripheral blood cells left untreated (orange histogram) or treated with hydrogen-peroxide-activated sodium pervanadate (purple histogram) and stained with 0.06 µg of Anti-Human/Mouse phospho-Tyrosine eFluor® 450 (purple histogram) using the IC Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Cells in the lymphocyte gate were used for analysis.

Mouse Mono [pY20], 100 Test
Hu, Mo
FACS

Supplier data
NSJ Bioreagents V3095-100UG Phosphotyrosine antibody, V3095-100UG, NSJ Bioreagents. Formalin-fixed, paraffin-embedded human breast carcinoma stained with Phosphotyrosine antibody (PY793).

Phosphotyrosine antibody, V3095-100UG, NSJ Bioreagents. Formalin-fixed, paraffin-embedded human breast carcinoma stained with Phosphotyrosine antibody (PY793).

Mouse Mono [PY793], 100 ug
Mam
FACS, IF, IHC-P

IF, IHC-P

Supplier data
NSJ Bioreagents V3095SAF-100UG Phosphotyrosine antibody, V3095SAF-100UG, NSJ Bioreagents. Formalin-fixed, paraffin-embedded human breast carcinoma stained with Phosphotyrosine antibody (PY793).

Phosphotyrosine antibody, V3095SAF-100UG, NSJ Bioreagents. Formalin-fixed, paraffin-embedded human breast carcinoma stained with Phosphotyrosine antibody (PY793).

Mouse Mono [PY793], 100 ug
Mam
FACS, IF, IHC-P

IF, IHC-P

Supplier data
Cell Signaling Technology 8954S Phosphotyrosine antibody, 8954S, Cell Signaling Technology.

Rabbit Mono [P-Tyr-1000], 100 ul
Bac, Bv, Cel, Ch, Cn, Dk, Dr, Du, Eq, Eu, Fe, Fi, Fr, Gf, GP, Gt, Ha, Hu, Inv, Mam, Mo, Pl, Po, Pr, Pro, Rb, Rt, Sh, Sq, Vert, Vi, Xe, Yt, Zf
FACS, ICC, IF, IP, WB

Phosphotyrosine antibody, 8954S, Cell Signaling Technology, Immunocytochemistry image

ICC,

Phosphotyrosine antibody, 8954S, Cell Signaling Technology, Immunofluorescence image

IF,

Phosphotyrosine antibody, 8954S, Cell Signaling Technology, Immunoprecipitation image

IP,

Western Blot

Supplier data
BioLegend 309301

Phosphotyrosine antibody, 309301, BioLegend. Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-phosphotyrosine antibody (clone PY-20). <br>Lane 1, serum-starved Hela cells; Lane 2, serum-starved Hela cells following serum addition for 4 hrs.<br> Lane 2 shows an upregulation of tyrosine phosphorylated proteins after serum addition. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.

Mouse Mono [PY20], 25 ug
Bac, Bv, Cel, Ch, Cn, Dk, Dr, Eq, Eu, Fe, Fi, Fr, Gf, GP, Gt, Ha, Hu, Mam, Mo, Pl, Po, Pr, Pro, Rb, Rt, Sh, Vi, Xe, Yt, Zf
FACS, IF, IP, WB

FACS, IF, IP

Supplier data
BioLegend 309302

Phosphotyrosine antibody, 309302, BioLegend. Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-phosphotyrosine antibody (clone PY-20). <br>Lane 1, serum-starved Hela cells; Lane 2, serum-starved Hela cells following serum addition for 4 hrs.<br> Lane 2 shows an upregulation of tyrosine phosphorylated proteins after serum addition. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.

Mouse Mono [PY20], 100 ug
Bac, Bv, Cel, Ch, Cn, Dk, Dr, Eq, Eu, Fe, Fi, Fr, Gf, GP, Gt, Ha, Hu, Mam, Mo, Pl, Po, Pr, Pro, Rb, Rt, Sh, Vi, Xe, Yt, Zf
FACS, IF, IP, WB

FACS, IF, IP

Supplier data
Invitrogen Antibodies 14-5001-82 Phosphotyrosine antibody, 14-5001-82, Invitrogen Antibodies. Immunoblotting of reduced lysates from mouse splenocytes left untreated (lane 1) or treated with hydrogen peroxide-activated sodium orthovanadate (lane 2), and probed with 1 µg/mL of Anti-Human/Mouse Phosphotyrosine Purified. Bands were visualized using Rat Anti-Mouse IgG HRP.

Phosphotyrosine antibody, 14-5001-82, Invitrogen Antibodies. Immunoblotting of reduced lysates from mouse splenocytes left untreated (lane 1) or treated with hydrogen peroxide-activated sodium orthovanadate (lane 2), and probed with 1 µg/mL of Anti-Human/Mouse Phosphotyrosine Purified. Bands were visualized using Rat Anti-Mouse IgG HRP.

Mouse Mono [pY20], 100 ug
Hu, Mo
FACS, IP, WB

FACS, IP

Supplier data
Invitrogen Antibodies 14-5001-80 Phosphotyrosine antibody, 14-5001-80, Invitrogen Antibodies. Immunoblotting of reduced lysates from mouse splenocytes left untreated (lane 1) or treated with hydrogen peroxide-activated sodium orthovanadate (lane 2), and probed with 1 µg/mL of Anti-Human/Mouse Phosphotyrosine Purified. Bands were visualized using Rat Anti-Mouse IgG HRP.

Phosphotyrosine antibody, 14-5001-80, Invitrogen Antibodies. Immunoblotting of reduced lysates from mouse splenocytes left untreated (lane 1) or treated with hydrogen peroxide-activated sodium orthovanadate (lane 2), and probed with 1 µg/mL of Anti-Human/Mouse Phosphotyrosine Purified. Bands were visualized using Rat Anti-Mouse IgG HRP.

Mouse Mono [pY20], 25 ug
Hu, Mo
FACS, IP, WB

FACS, IP

Supplier data
NSJ Bioreagents V2359SAF-100UG Phosphotyrosine antibody, V2359SAF-100UG, NSJ Bioreagents. IHC staining of breast carcinoma with phosphotyrosine antibody (PY20). Note cell surface and cytoplasmic staining.

Phosphotyrosine antibody, V2359SAF-100UG, NSJ Bioreagents. IHC staining of breast carcinoma with phosphotyrosine antibody (PY20). Note cell surface and cytoplasmic staining.

Mouse Mono [PY20], 100 ug
Mam
FACS, IF, IHC-F, IHC-P, IP, WB

FACS, IF, IHC-F, IHC-P, IP

Supplier data
NSJ Bioreagents V2359-100UG Phosphotyrosine antibody, V2359-100UG, NSJ Bioreagents. IHC staining of breast carcinoma with phosphotyrosine antibody (PY20). Note cell surface and cytoplasmic staining.

Phosphotyrosine antibody, V2359-100UG, NSJ Bioreagents. IHC staining of breast carcinoma with phosphotyrosine antibody (PY20). Note cell surface and cytoplasmic staining.

Mouse Mono [PY20], 100 ug
Mam
FACS, IF, IHC-F, IHC-P, IP, WB

FACS, IF, IHC-F, IHC-P, IP

Supplier data
Cell Signaling Technology 8954S Phosphotyrosine antibody, 8954S, Cell Signaling Technology.

Rabbit Mono [P-Tyr-1000], 100 ul
Bac, Bv, Cel, Ch, Cn, Dk, Dr, Du, Eq, Eu, Fe, Fi, Fr, Gf, GP, Gt, Ha, Hu, Inv, Mam, Mo, Pl, Po, Pr, Pro, Rb, Rt, Sh, Sq, Vert, Vi, Xe, Yt, Zf
FACS, ICC, IF, IP, WB

Phosphotyrosine antibody, 8954S, Cell Signaling Technology, Flow Cytometry image

FACS,

ICC,

IF,

Supplier data
ProSci 33-259 Phosphotyrosine antibody, 33-259, ProSci. IHC staining of breast carcinoma with phosphotyrosine antibody (PY20). Note cell surface and cytoplasmic staining.

Phosphotyrosine antibody, 33-259, ProSci. IHC staining of breast carcinoma with phosphotyrosine antibody (PY20). Note cell surface and cytoplasmic staining.

Mouse Mono [PY20], 100 ug
Ch
FACS, IF, IHC-F, IHC-P, IP, WB

FACS, IF, IHC-F, IHC-P, IP

Immunofluorescence

Supplier data
Cell Signaling Technology 8954S Phosphotyrosine antibody, 8954S, Cell Signaling Technology.

Rabbit Mono [P-Tyr-1000], 100 ul
Bac, Bv, Cel, Ch, Cn, Dk, Dr, Du, Eq, Eu, Fe, Fi, Fr, Gf, GP, Gt, Ha, Hu, Inv, Mam, Mo, Pl, Po, Pr, Pro, Rb, Rt, Sh, Sq, Vert, Vi, Xe, Yt, Zf
FACS, ICC, IF, IP, WB

FACS,

ICC,

IP,

Immunoprecipitation

Supplier data
Cell Signaling Technology 8954S Phosphotyrosine antibody, 8954S, Cell Signaling Technology.

Rabbit Mono [P-Tyr-1000], 100 ul
Bac, Bv, Cel, Ch, Cn, Dk, Dr, Du, Eq, Eu, Fe, Fi, Fr, Gf, GP, Gt, Ha, Hu, Inv, Mam, Mo, Pl, Po, Pr, Pro, Rb, Rt, Sh, Sq, Vert, Vi, Xe, Yt, Zf
FACS, ICC, IF, IP, WB

FACS,

ICC,

IF,

Phosphotyrosine antibodies are often published with	Associated citations
Erk1	13.3%
Pleckstrin And Sec7 Domain Containing	6.7%
Insulin Like Growth Factor 1 Receptor	6.7%
Insulin Like Growth Factor 1	6.7%
Heat Shock Protein Family B (Small) Member 1	6.7%
Serpin Family B Member 5	6.7%
Zyxin	6.7%
ENAH Actin Regulator	6.7%
Kallikrein B1	6.7%
C-Type Lectin Domain Family 1 Member B	6.7%

Phosphotyrosine antibodies are often published with

Associated citations

Erk1

13.3%

Pleckstrin And Sec7 Domain Containing

6.7%

Insulin Like Growth Factor 1 Receptor

6.7%

Insulin Like Growth Factor 1

6.7%

Heat Shock Protein Family B (Small) Member 1

6.7%

Serpin Family B Member 5

6.7%

Zyxin

6.7%

ENAH Actin Regulator

6.7%

Kallikrein B1

6.7%

C-Type Lectin Domain Family 1 Member B

6.7%

All None
	WB	IF	ICC	IP	ELISA	FACS	IHC-F	IHC-P	AP	DB
Human	146	81	86	102	91	85	57	59	6	1
Chicken	130	122	120	65	80	81	63	60	1
Mouse	125	70	71	92	80	73	44	44	6
Rat	118	70	71	87	77	51	44	44	6
Canine	99	59	56	75	68	43	40	37	6
Mammals	91	60	60	49	42	49	43	43	1
Hamster	67	54	55	44	39	40	37	36
Porcine	66	56	57	42	41	42	39	38
Bacteria	66	56	56	42	40	45	38	38
Bovine	66	54	56	42	39	40	37	36
Sheep	65	54	55	42	39	40	37	36
Xenopus	65	54	55	42	39	40	37	36
Drosophila	65	54	55	42	39	40	37	36
Plant	65	54	55	42	39	40	37	36
Fish	65	54	55	42	39	40	37	36
Eukaryotes	65	54	55	42	39	40	37	36
Primate	65	54	55	42	39	40	37	36
Goat	65	54	55	42	39	40	37	36
Goldfish	65	54	55	42	39	40	37	36
Yeast	65	54	55	42	39	40	37	36
Zebrafish	65	55	56	42	40	41	38	37
Donkey	65	54	55	42	39	40	37	36
C.Elegans	65	54	55	42	39	40	37	36
Feline	65	55	56	42	40	41	38	37
Prokaryotes	65	54	55	42	39	40	37	36
Guinea Pig	65	54	55	42	39	40	37	36
Virus	65	54	55	42	39	40	37	36
Rabbit	65	55	56	42	40	41	38	37
Equine	65	54	55	42	39	40	37	36
Invertebrates	50	47	46	37	35	35	36	36
Squirrel	50	47	46	37	35	35	36	36
Duck	50	47	46	37	35	35	36	36
Vertebrates	50	47	46	37	35	35	36	36
Fungus	44	43	43	35	34	34	34	34
Frog	27	13	12	12	5	7	5	2
Chemical	15	4		9	15	1

Bac	Bacteria
Bt	Bat
Bv	Bovine
Cel	C.Elegans
Cml	Camel
Cn	Canine
chem	Chemical
Ch	Chicken
Dk	Donkey
Dr	Drosophila
Du	Duck
Eq	Equine
Eu	Eukaryotes
Fe	Feline
Fi	Fish
Fr	Frog
Fu	Fungus
Gt	Goat
Gf	Goldfish
GP	Guinea Pig
Ha	Hamster
Hu	Human
Inv	Invertebrates
Llm	Llama
Mam	Mammals
Mo	Mouse
Pl	Plant
Po	Porcine
Pr	Primate
Pro	Prokaryotes
Rb	Rabbit
Rt	Rat
Sh	Sheep
Sq	Squirrel
Vert	Vertebrates
Vi	Virus
Xe	Xenopus
Yt	Yeast
Zf	Zebrafish

AP	Affinity purification
Bl	Blocking
ChIP	Chromatin Immunoprecipitation
DB	Dot Blot
EMSA	Electron Microscopy
ELISA	Enzyme Linked Immunosorbent Assay
FACS	Flow Cytometry
FA	Functional Assay
GS	Gel Shift
ICC	Immunocytochemistry
ID	Immunodiffusion
IF	Immunofluorescence
IHC-F	Immunohistochemistry frozen
IHC-P	Immunohistochemistry paraffin
IP	Immunoprecipitation
IVT	In vivo targeting
Neu	Neutralising
Pep-ELISA	Peptide ELISA
PLA	Proximity Ligation Assay
RIA	Radioimmunoassay
WB	Western Blot

105 matching Phosphotyrosine antibodies for Flow Cytometry from 27 suppliers

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105 matching Phosphotyrosine antibodies for Flow Cytometry after applying filters

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