Growth Arrest And DNA Damage Inducible Alpha Antibodies for Western Blot

105 matching Growth Arrest And DNA Damage Inducible Alpha antibodies for Western Blot after applying filters

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Supplier data
FineTest FNab03294 Growth Arrest And DNA Damage Inducible Alpha antibody, FNab03294, FineTest.

Rabbit Poly, 100 ug
Hu, Mo, Rt
ELISA, WB

ELISA

Supplier data
Origene TA507380S Growth Arrest And DNA Damage Inducible Alpha antibody, TA507380S, Origene.

Mouse Mono [OTI2D5], 30 ul
Hu, Mo, Rt
WB

Supplier data
Origene TA507370S Growth Arrest And DNA Damage Inducible Alpha antibody, TA507370S, Origene.

Mouse Mono [OTI4D4], 30 ul
Hu, Mo, Rt
WB

Supplier data
Origene TA332434 Growth Arrest And DNA Damage Inducible Alpha antibody, TA332434, Origene.

Rabbit Poly, 100 ul
Hu, Mo, Rt
IF, IHC-F, IHC-P, WB

IF, IHC-F, IHC-P

Supplier data
Origene TA507370 Growth Arrest And DNA Damage Inducible Alpha antibody, TA507370, Origene.

Mouse Mono [OTI1C9], 100 ul
Hu
WB

Supplier data
Origene CF507370 Growth Arrest And DNA Damage Inducible Alpha antibody, CF507370, Origene.

Mouse Mono [Clone OTI1C9], 100 ug
Hu
WB

Supplier data
Proteintech Group 13747-1-AP Growth Arrest And DNA Damage Inducible Alpha antibody, 13747-1-AP, Proteintech Group.

Rabbit Poly, 150 ul
Hu, Mo, Rt
ELISA, IP, WB

ELISA,

Growth Arrest And DNA Damage Inducible Alpha antibody, 13747-1-AP, Proteintech Group, Immunoprecipitation image

Supplier data
Novus Biologicals NBP1-79791 Growth Arrest And DNA Damage Inducible Alpha antibody, NBP1-79791, Novus Biologicals. Western Blot: GADD45A Antibody [NBP1-79791] - Human Heart lysate; concentration 0.2-1 ug/ml.

Growth Arrest And DNA Damage Inducible Alpha antibody, NBP1-79791, Novus Biologicals. Western Blot: GADD45A Antibody [NBP1-79791] - Human Heart lysate; concentration 0.2-1 ug/ml.

Rabbit Poly, 50 ug
Hu, Po
WB

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Origene TA342868 Growth Arrest And DNA Damage Inducible Alpha antibody, TA342868, Origene.

Rabbit Poly, 50 ug
Hu
WB

Supplier data
Origene TA507380 Growth Arrest And DNA Damage Inducible Alpha antibody, TA507380, Origene.

Mouse Mono [OTI2B7], 100 ul
Hu
WB

Supplier data
Origene AP18028PU-N Growth Arrest And DNA Damage Inducible Alpha antibody, AP18028PU-N, Origene.

Rabbit Poly, 400 ul
Hu
WB

Supplier data
Origene AP12434PU-N Growth Arrest And DNA Damage Inducible Alpha antibody, AP12434PU-N, Origene.

Rabbit Poly, 400 ul
Hu, Mo
WB

Supplier data
Origene CF507380 Growth Arrest And DNA Damage Inducible Alpha antibody, CF507380, Origene.

Mouse Mono [Clone OTI2B7], 100 ug
Hu
WB

Supplier data
Novus Biologicals H00001647-B01P Growth Arrest And DNA Damage Inducible Alpha antibody, H00001647-B01P, Novus Biologicals. Western Blot: GADD45 alpha Antibody [H00001647-B01P] - Analysis of GADD45A expression in transfected 293T cell line by GADD45A polyclonal antibody. Lane 1: GADD45A transfected lysate(18.30 KDa). Lane 2: Non-transfected lysate.

Growth Arrest And DNA Damage Inducible Alpha antibody, H00001647-B01P, Novus Biologicals. Western Blot: GADD45 alpha Antibody [H00001647-B01P] - Analysis of GADD45A expression in transfected 293T cell line by GADD45A polyclonal antibody. Lane 1: GADD45A transfected lysate(18.30 KDa). Lane 2: Non-transfected lysate.

Mouse Poly, 50 ug
Hu
ELISA, WB

ELISA

Supplier data
Novus Biologicals NBP2-45806 Growth Arrest And DNA Damage Inducible Alpha antibody, NBP2-45806, Novus Biologicals. Western Blot: GADD45 alpha Antibody (1C9) [NBP2-45806] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45A.

Growth Arrest And DNA Damage Inducible Alpha antibody, NBP2-45806, Novus Biologicals. Western Blot: GADD45 alpha Antibody (1C9) [NBP2-45806] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45A.

Mouse Mono [1C9], 100 ul
Hu
WB

Supplier data
MyBioSource MBS125069 Growth Arrest And DNA Damage Inducible Alpha antibody, MBS125069, MyBioSource. Western blot analysis of extracts of COLO 320 cells tissue, using GADD45A antibody.

Growth Arrest And DNA Damage Inducible Alpha antibody, MBS125069, MyBioSource. Western blot analysis of extracts of COLO 320 cells tissue, using GADD45A antibody.

Rabbit Poly, 50 ul
Hu, Mo, Rt
WB

Supplier data
MyBioSource MBS9204989 Growth Arrest And DNA Damage Inducible Alpha antibody, MBS9204989, MyBioSource. Western blot analysis of anti-GADD45A Antibody (N-term) in Y79 cell line lysates (35ug/lane). GADD45A (arrow) was detected using the purified Pab.

Growth Arrest And DNA Damage Inducible Alpha antibody, MBS9204989, MyBioSource. Western blot analysis of anti-GADD45A Antibody (N-term) in Y79 cell line lysates (35ug/lane). GADD45A (arrow) was detected using the purified Pab.

Rabbit Poly, 80 ul
Hu
ELISA, FACS, WB

ELISA, FACS

Supplier data
MyBioSource MBS9206054 Growth Arrest And DNA Damage Inducible Alpha antibody, MBS9206054, MyBioSource. Western blot analysis of GADD45A Antibody (C-term) in Y79 cell line lysates (35ug/lane). GADD45A (arrow) was detected using the purified Pab.

Growth Arrest And DNA Damage Inducible Alpha antibody, MBS9206054, MyBioSource. Western blot analysis of GADD45A Antibody (C-term) in Y79 cell line lysates (35ug/lane). GADD45A (arrow) was detected using the purified Pab.

Rabbit Poly, 80 ul
Hu
ELISA, FACS, WB

ELISA, FACS

Supplier data
St John's Laboratory STJ23740 Growth Arrest And DNA Damage Inducible Alpha antibody, STJ23740, St John

Rabbit Poly, 100 ul
Hu, Mo, Rt
WB

Supplier data
St John's Laboratory STJ113355 Growth Arrest And DNA Damage Inducible Alpha antibody, STJ113355, St John

Rabbit Poly, 100 ul
Hu
WB

Supplier data
R&D Systems AF5510 Growth Arrest And DNA Damage Inducible Alpha antibody, AF5510, R&D Systems. Detection of Human GADD45 alpha by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line either mock transfected or transfected with full length human GADD45 alpha . PVDF membrane was probed with 1

Growth Arrest And DNA Damage Inducible Alpha antibody, AF5510, R&D Systems. Detection of Human GADD45 alpha by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line either mock transfected or transfected with full length human GADD45 alpha . PVDF membrane was probed with 1

Sheep Poly, 100 ug
Hu
WB

Supplier data
Boster Biological Technology PA1402 Growth Arrest And DNA Damage Inducible Alpha antibody, PA1402, Boster Biological Technology. Anti-GADD45A antibody, PA1402, Western blotting<br>Lane 1: MM453 Cell Lysate<br>Lane 2: MM231 Cell Lysate<br>Lane 3: HELA Cell Lysate<br>Lane 4: RAJI Cell Lysate<br>Lane 5: SW620 Cell Lysate<br>

Growth Arrest And DNA Damage Inducible Alpha antibody, PA1402, Boster Biological Technology. Anti-GADD45A antibody, PA1402, Western blotting<br>Lane 1: MM453 Cell Lysate<br>Lane 2: MM231 Cell Lysate<br>Lane 3: HELA Cell Lysate<br>Lane 4: RAJI Cell Lysate<br>Lane 5: SW620 Cell Lysate<br>

Rabbit Poly, 100 ug
Hu, Mo, Rt
WB

Supplier data
Boster Biological Technology PB9945 Growth Arrest And DNA Damage Inducible Alpha antibody, PB9945, Boster Biological Technology. Figure 1. Western blot analysis of GADD45A using anti-GADD45A antibody (PB9945). <br>

Growth Arrest And DNA Damage Inducible Alpha antibody, PB9945, Boster Biological Technology. Figure 1. Western blot analysis of GADD45A using anti-GADD45A antibody (PB9945). <br>

Rabbit Poly, 100 ug
Hu
WB

Supplier data
Boster Biological Technology A01806 Growth Arrest And DNA Damage Inducible Alpha antibody, A01806, Boster Biological Technology. <h4>Figure 1. Western blotting validation for Anti-GADD45A Antibody A01806</h4>

Growth Arrest And DNA Damage Inducible Alpha antibody, A01806, Boster Biological Technology. <h4>Figure 1. Western blotting validation for Anti-GADD45A Antibody A01806</h4>

Rabbit Poly, 100 ul
Hu, Mo
IHC-F, IHC-P, WB

IHC-F, IHC-P

Supplier data
Boster Biological Technology M01806 Growth Arrest And DNA Damage Inducible Alpha antibody, M01806, Boster Biological Technology. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45A.

Growth Arrest And DNA Damage Inducible Alpha antibody, M01806, Boster Biological Technology. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45A.

Mouse Mono [OTI1C9], 100 ul
Hu
WB

Supplier data
ABclonal Technology A1797 Growth Arrest And DNA Damage Inducible Alpha antibody, A1797, ABclonal Technology. Western blot analysis of extracts of various cell lines, using GADD45A antibody.

Growth Arrest And DNA Damage Inducible Alpha antibody, A1797, ABclonal Technology. Western blot analysis of extracts of various cell lines, using GADD45A antibody.

Rabbit Poly, 200 ul
Bac, Hu, Mo, Rb
IF, IHC-F, IHC-P, WB

Growth Arrest And DNA Damage Inducible Alpha antibody, A1797, ABclonal Technology, Immunofluorescence image

IF, IHC-F, IHC-P

Supplier data
ABclonal Technology A0836 Growth Arrest And DNA Damage Inducible Alpha antibody, A0836, ABclonal Technology. Western blot analysis of extracts of COLO 320 cells tissue, using GADD45A antibody.

Growth Arrest And DNA Damage Inducible Alpha antibody, A0836, ABclonal Technology. Western blot analysis of extracts of COLO 320 cells tissue, using GADD45A antibody.

Rabbit Poly, 200 ul
Bac, Hu, Mo, Rb
IP, WB

Supplier data
Lifespan Biosciences LS-B14869 Growth Arrest And DNA Damage Inducible Alpha antibody, LS-B14869, Lifespan Biosciences. Western blot analysis of extracts of various cell lines, using GADD45A antibody.

Growth Arrest And DNA Damage Inducible Alpha antibody, LS-B14869, Lifespan Biosciences. Western blot analysis of extracts of various cell lines, using GADD45A antibody.

Rabbit Poly, 1 Vial
Hu, Mo, Rt
IF, IHC-F, IHC-P, WB

Growth Arrest And DNA Damage Inducible Alpha antibody, LS-B14869, Lifespan Biosciences, Immunofluorescence image

IF,

Growth Arrest And DNA Damage Inducible Alpha antibody, LS-B14869, Lifespan Biosciences, Immunohistochemistry frozen image

IHC-F,

Growth Arrest And DNA Damage Inducible Alpha antibody, LS-B14869, Lifespan Biosciences, Immunohistochemistry paraffin image

IHC-P

Supplier data
Lifespan Biosciences LS-B13744 Growth Arrest And DNA Damage Inducible Alpha antibody, LS-B13744, Lifespan Biosciences. Western blot of GADD45A (arrow) using rabbit polyclonal GADD45A Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GADD45A gene.

Growth Arrest And DNA Damage Inducible Alpha antibody, LS-B13744, Lifespan Biosciences. Western blot of GADD45A (arrow) using rabbit polyclonal GADD45A Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GADD45A gene.

Rabbit Poly, 1 Vial
Hu
FACS, IHC-P, WB

Growth Arrest And DNA Damage Inducible Alpha antibody, LS-B13744, Lifespan Biosciences, Flow Cytometry image

FACS,

Growth Arrest And DNA Damage Inducible Alpha antibody, LS-B13744, Lifespan Biosciences, Immunohistochemistry paraffin image

IHC-P

Supplier data
Lifespan Biosciences LS-C166334 Growth Arrest And DNA Damage Inducible Alpha antibody, LS-C166334, Lifespan Biosciences. Western blot of GADD45A (arrow) using rabbit polyclonal GADD45A Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GADD45A gene.

Growth Arrest And DNA Damage Inducible Alpha antibody, LS-C166334, Lifespan Biosciences. Western blot of GADD45A (arrow) using rabbit polyclonal GADD45A Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GADD45A gene.

Rabbit Poly, 1 Vial
Hu
FACS, WB

Growth Arrest And DNA Damage Inducible Alpha antibody, LS-C166334, Lifespan Biosciences, Flow Cytometry image

FACS

Supplier data
Lifespan Biosciences LS-C747126 Growth Arrest And DNA Damage Inducible Alpha antibody, LS-C747126, Lifespan Biosciences. Western blot analysis of extracts of various cell lines, using GADD45A antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBS ...

Growth Arrest And DNA Damage Inducible Alpha antibody, LS-C747126, Lifespan Biosciences. Western blot analysis of extracts of various cell lines, using GADD45A antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBS ...

Rabbit Poly, 1 Vial
Hu
WB

Supplier data
Lifespan Biosciences LS-C748536 Growth Arrest And DNA Damage Inducible Alpha antibody, LS-C748536, Lifespan Biosciences. Western blot analysis of extracts of mouse liver, using GADD45A antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was u ...

Growth Arrest And DNA Damage Inducible Alpha antibody, LS-C748536, Lifespan Biosciences. Western blot analysis of extracts of mouse liver, using GADD45A antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was u ...

Rabbit Poly, 1 Vial
Hu, Mo, Rt
WB

Supplier data
Lifespan Biosciences LS-C812977 Growth Arrest And DNA Damage Inducible Alpha antibody, LS-C812977, Lifespan Biosciences. Western blot detection of GADD45 a in CHO-K1 cell lysate (B) and CHO-K1 transfected by GADD45 a His fusion protein (A) cell lysate using GADD45 a mouse mAb (1:1000 diluted). Predicted band size: 22KDa. Observed band size: 22KDa.

Growth Arrest And DNA Damage Inducible Alpha antibody, LS-C812977, Lifespan Biosciences. Western blot detection of GADD45 a in CHO-K1 cell lysate (B) and CHO-K1 transfected by GADD45 a His fusion protein (A) cell lysate using GADD45 a mouse mAb (1:1000 diluted). Predicted band size: 22KDa. Observed band size: 22KDa.

Mouse Mono [LS-C812977], 1 Vial
Hu
ELISA, WB

ELISA

Supplier data
Lifespan Biosciences LS-C788579 Growth Arrest And DNA Damage Inducible Alpha antibody, LS-C788579, Lifespan Biosciences. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45A.

Growth Arrest And DNA Damage Inducible Alpha antibody, LS-C788579, Lifespan Biosciences. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45A.

Mouse Mono [LS-C788579], 1 Vial
Hu
WB

Supplier data
Lifespan Biosciences LS-C788573 Growth Arrest And DNA Damage Inducible Alpha antibody, LS-C788573, Lifespan Biosciences. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45A.

Growth Arrest And DNA Damage Inducible Alpha antibody, LS-C788573, Lifespan Biosciences. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45A.

Mouse Mono [LS-C788573], 1 Vial
Hu
WB

Supplier data
Lifespan Biosciences LS-C338484 Growth Arrest And DNA Damage Inducible Alpha antibody, LS-C338484, Lifespan Biosciences. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45A.

Growth Arrest And DNA Damage Inducible Alpha antibody, LS-C338484, Lifespan Biosciences. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45A.

Mouse Mono [OTI1C9], 1 Vial
Hu
WB

Supplier data
Lifespan Biosciences LS-C338488 Growth Arrest And DNA Damage Inducible Alpha antibody, LS-C338488, Lifespan Biosciences. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45A.

Growth Arrest And DNA Damage Inducible Alpha antibody, LS-C338488, Lifespan Biosciences. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45A.

Mouse Mono [OTI2B7], 1 Vial
Hu
WB

Supplier data
Lifespan Biosciences LS-C373751 Growth Arrest And DNA Damage Inducible Alpha antibody, LS-C373751, Lifespan Biosciences. Western blot of GADD45A / GADD45 antibody.

Growth Arrest And DNA Damage Inducible Alpha antibody, LS-C373751, Lifespan Biosciences. Western blot of GADD45A / GADD45 antibody.

Rabbit Poly, 1 Vial
Rt
WB

Supplier data
Lifespan Biosciences LS-C373749 Growth Arrest And DNA Damage Inducible Alpha antibody, LS-C373749, Lifespan Biosciences. Western Blot; Lane1: Human Placenta Tissue; Lane2: Mouse Liver Tissue; Lane3: Mouse Kidney Tissue; Lane4: Mouse Lung Tissue; Lane5: Mouse Spleen Tissue.

Growth Arrest And DNA Damage Inducible Alpha antibody, LS-C373749, Lifespan Biosciences. Western Blot; Lane1: Human Placenta Tissue; Lane2: Mouse Liver Tissue; Lane3: Mouse Kidney Tissue; Lane4: Mouse Lung Tissue; Lane5: Mouse Spleen Tissue.

Rabbit Poly, 1 Vial
Hu, Mo
WB

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Bac	Bacteria
Bt	Bat
Bv	Bovine
Cel	C.Elegans
Cml	Camel
Cn	Canine
chem	Chemical
Ch	Chicken
Dk	Donkey
Dr	Drosophila
Du	Duck
Eq	Equine
Eu	Eukaryotes
Fe	Feline
Fi	Fish
Fr	Frog
Fu	Fungus
Gt	Goat
Gf	Goldfish
GP	Guinea Pig
Ha	Hamster
Hu	Human
Inv	Invertebrates
Llm	Llama
Mam	Mammals
Mo	Mouse
Pl	Plant
Po	Porcine
Pr	Primate
Pro	Prokaryotes
Rb	Rabbit
Rt	Rat
Sh	Sheep
Sq	Squirrel
Vert	Vertebrates
Vi	Virus
Xe	Xenopus
Yt	Yeast
Zf	Zebrafish

AP	Affinity purification
Bl	Blocking
ChIP	Chromatin Immunoprecipitation
DB	Dot Blot
EMSA	Electron Microscopy
ELISA	Enzyme Linked Immunosorbent Assay
FACS	Flow Cytometry
FA	Functional Assay
GS	Gel Shift
ICC	Immunocytochemistry
ID	Immunodiffusion
IF	Immunofluorescence
IHC-F	Immunohistochemistry frozen
IHC-P	Immunohistochemistry paraffin
IP	Immunoprecipitation
IVT	In vivo targeting
Neu	Neutralising
Pep-ELISA	Peptide ELISA
PLA	Proximity Ligation Assay
RIA	Radioimmunoassay
WB	Western Blot

105 matching Growth Arrest And DNA Damage Inducible Alpha antibodies for Western Blot from 16 suppliers

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Growth Arrest And DNA Damage Inducible Alpha information

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105 matching Growth Arrest And DNA Damage Inducible Alpha antibodies for Western Blot after applying filters

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Related antibodies

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	WB	ELISA	IF	IHC-P	FACS	ICC	IHC-F	IP	AP	Pep-ELISA	PLA
Human	151	67	51	45	22	21	20	14	7	4	1
Mouse	95	24	35	36	9	17	19	7	5	4
Rat	60	17	31	33	8	18	17	8	2	4
Primate	6
Rabbit	3		1	2			1	1
Bovine	3			1
Bacteria	3		1	2			2	1
Porcine	3			1
Bat	1			1
Chicken				1			1