AKT1 Substrate 1 Antibodies for Flow Cytometry

0 matching AKT1 Substrate 1 antibodies for Flow Cytometry after applying filters

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Supplier data
Invitrogen Antibodies 710089

AKT1 Substrate 1 antibody, 710089, Invitrogen Antibodies. Flow cytometry analysis of PRAS40 [pT246] was done on U-87 MG cells treated with PDGF (100 ng/ml, 15 minutes). Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton? X-100 for 20 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were labeled with ABfinity? PRAS40 [pT246] Recombinant Rabbit Oligoclonal Antibody (710089, red histogram) or with rabbit isotype control (pink histogram) at a dilution of 1:250 in 2.5% BSA. After incubation at room temper

Rabbit Poly, 100 ug
Hu, Mo
FACS, IF, WB

AKT1 Substrate 1 antibody, 710089, Invitrogen Antibodies, Immunofluorescence image

IF,

AKT1 Substrate 1 antibody, 710089, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 701058

AKT1 Substrate 1 antibody, 701058, Invitrogen Antibodies. Flow cytometry analysis of PRAS40 (pT246) was performed on U-87 MG cells treated with PDGF (100 ng/ml, 15 minutes). Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0. 25% Triton? X-100 for 20 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were labeled with ABfinity? PRAS40 (pT246) recombinant rabbit monoclonal antibody (Product # 701058, red histogram) or with rabbit isotype control (pink histogram) at a dilution of 1:250 in 2.5% BSA. After incubation

Rabbit Mono [701058], 100 ug
Hu
FACS, IF, WB

AKT1 Substrate 1 antibody, 701058, Invitrogen Antibodies, Immunofluorescence image

IF,

AKT1 Substrate 1 antibody, 701058, Invitrogen Antibodies, Western Blot image

Supplier data
Biorbyt orb304683 AKT1 Substrate 1 antibody, orb304683, Biorbyt. Flow cytometric analysis of PARD3 antibody in k562 cell line lysates

AKT1 Substrate 1 antibody, orb304683, Biorbyt. Flow cytometric analysis of PARD3 antibody in k562 cell line lysates

Rabbit Poly, 200 ul
Hu, Mo, Rt
FACS, IHC-P, WB

AKT1 Substrate 1 antibody, orb304683, Biorbyt, Immunohistochemistry paraffin image

IHC-P,

Western Blot

Supplier data
Boster Biological Technology M03629 AKT1 Substrate 1 antibody, M03629, Boster Biological Technology. Western blot analysis of PRAS40 expression in 293T cell lysate (M03629) in 293T cell lysate. <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> After Electrophoresis

Rabbit Mono [M03629], 100 ug
Hu, Mo
FACS, IHC-F, IHC-P, IP, WB

FACS, IHC-F, IHC-P, IP

Supplier data
Invitrogen Antibodies 710089

AKT1 Substrate 1 antibody, 710089, Invitrogen Antibodies. Western blot analysis of Phospho-AKT1S1 pThr246 in whole cell extracts of NIH-3T3 cells treated with 100 ng/mL PDGF for 15 min using a Phospho-AKT1S1 pThr246 recombinant rabbit oligoclonal antibody (Product # 710089) at a dilution of 2.5 µg/mL. To confirm specificity, competition was performed by preincubation with the phosphopeptide to inhibit antibody binding (lane 2). Results show a band at ~40 kDa.

Rabbit Poly, 100 ug
Hu, Mo
FACS, IF, WB

AKT1 Substrate 1 antibody, 710089, Invitrogen Antibodies, Flow Cytometry image

FACS,

Supplier data
Invitrogen Antibodies 701058

AKT1 Substrate 1 antibody, 701058, Invitrogen Antibodies. Western blot analysis of Phospho-AKT1S1 pThr246 in whole cell extracts of HepG2 cells treated with PDGF (100 ng/mL, 15 min) using a Phospho-AKT1S1 pThr246 recombinant rabbit monoclonal antibody (Product # 701058) at a dilution of 2.5 µg/mL. To confirm specificity, competition was performed by preincubation with the phosphopeptide to inhibit antibody binding (lane 1). Results show a band at ~40 kDa.

Rabbit Mono [701058], 100 ug
Hu
FACS, IF, WB

AKT1 Substrate 1 antibody, 701058, Invitrogen Antibodies, Flow Cytometry image

FACS,

Supplier data
Biorbyt orb304683 AKT1 Substrate 1 antibody, orb304683, Biorbyt. Western blot analysis of MCF7 (Lane 1), human Testis (Lane 2) whole cell lysates using PRAS40 antibody, Immunohistochemical analysis of formalin-fixed and paraffin-embedded human breast cancer tissue using PRAS40 antibody

AKT1 Substrate 1 antibody, orb304683, Biorbyt. Western blot analysis of MCF7 (Lane 1), human Testis (Lane 2) whole cell lysates using PRAS40 antibody, Immunohistochemical analysis of formalin-fixed and paraffin-embedded human breast cancer tissue using PRAS40 antibody

Rabbit Poly, 200 ul
Hu, Mo, Rt
FACS, IHC-P, WB

AKT1 Substrate 1 antibody, orb304683, Biorbyt, Flow Cytometry image

FACS,

IHC-P

Immunohistochemistry paraffin

Supplier data
Biorbyt orb304683 AKT1 Substrate 1 antibody, orb304683, Biorbyt.

Rabbit Poly, 200 ul
Hu, Mo, Rt
FACS, IHC-P, WB

FACS,

Immunofluorescence

Supplier data
Invitrogen Antibodies 710089 AKT1 Substrate 1 antibody, 710089, Invitrogen Antibodies. Immunofluorescent analysis of PRAS40 (pT246) was done by treating serum starved HeLa cells with 20 ng/mL PDGF for 15 minutes. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton

AKT1 Substrate 1 antibody, 710089, Invitrogen Antibodies. Immunofluorescent analysis of PRAS40 (pT246) was done by treating serum starved HeLa cells with 20 ng/mL PDGF for 15 minutes. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton

Rabbit Poly, 100 ug
Hu, Mo
FACS, IF, WB

FACS,

Supplier data
Invitrogen Antibodies 701058 AKT1 Substrate 1 antibody, 701058, Invitrogen Antibodies. Immunofluorescent analysis of PRAS40 (pT246) was done by treating serum starved U-87 MG cells with 20 ng/mL PDGF for 15 minutes. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton

AKT1 Substrate 1 antibody, 701058, Invitrogen Antibodies. Immunofluorescent analysis of PRAS40 (pT246) was done by treating serum starved U-87 MG cells with 20 ng/mL PDGF for 15 minutes. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton

Rabbit Mono [701058], 100 ug
Hu
FACS, IF, WB

FACS,

AKT1 Substrate 1 antibodies are often published with	Associated citations
Akt	41.7%
Distal-Less Homeobox 4	16.7%
Regulatory Associated Protein Of MTOR Complex 1	12.5%
Eukaryotic Translation Initiation Factor 4E Binding Protein 1	12.5%
GAPDH	12.5%
N-Myc Downstream Regulated 1	12.5%
VPS39 Subunit Of HOPS Complex	8.3%
RPTOR Independent Companion Of MTOR Complex 2	8.3%
Mechanistic Target Of Rapamycin Kinase	8.3%
Transcription Factor 3	8.3%

AKT1 Substrate 1 antibodies are often published with

Associated citations

Akt

41.7%

Distal-Less Homeobox 4

16.7%

Regulatory Associated Protein Of MTOR Complex 1

12.5%

Eukaryotic Translation Initiation Factor 4E Binding Protein 1

12.5%

GAPDH

12.5%

N-Myc Downstream Regulated 1

12.5%

VPS39 Subunit Of HOPS Complex

8.3%

RPTOR Independent Companion Of MTOR Complex 2

8.3%

Mechanistic Target Of Rapamycin Kinase

8.3%

Transcription Factor 3

8.3%

All None
	WB	IHC-P	ELISA	IHC-F	IP	IF	ICC	AP	Pep-ELISA	FACS
Human	225	101	86	69	36	31	18	14	5	4
Mouse	125	58	31	31	21	23	13	1	5	3
Rat	100	52	32	22	21	20	12		5	1
Primate	28	13	1		15				1
Bovine	27	7	3	3	5				2
Guinea Pig	13	4	3		5				1
Equine	12	4	3		5				2
Hamster	11	4	1		6				1
Canine	9	4	1	1	5				2
Drosophila	6	2	1	1	3				1
Porcine	6	2	1	1	3				1
Mammals	5	2	1		3				1
Sheep	5	3	3		3				1
Bacteria	4	2	1	1	3				1
Bat	4
Rabbit	4	1	1		3				1
Fish	3	1	1		3				1
C.Elegans	3	1	1		3				1
Frog	3	1	1		3				1
Goldfish	3	1	1		3				1
Feline	3	1	1		3				1
Yeast	3	1	1		3				1
Virus	3	1	1		3				1
Chicken	3	1	1		3				1
Plant	3	1	1		3				1
Zebrafish	3	1	1		3				1
Xenopus	3	1	1		3				1
Prokaryotes	3	1	1		3				1
Donkey	3	1	1		3				1
Eukaryotes	3	1	1		3				1
Goat	3	1	1		3				1

Bac	Bacteria
Bt	Bat
Bv	Bovine
Cel	C.Elegans
Cml	Camel
Cn	Canine
chem	Chemical
Ch	Chicken
Dk	Donkey
Dr	Drosophila
Du	Duck
Eq	Equine
Eu	Eukaryotes
Fe	Feline
Fi	Fish
Fr	Frog
Fu	Fungus
Gt	Goat
Gf	Goldfish
GP	Guinea Pig
Ha	Hamster
Hu	Human
Inv	Invertebrates
Llm	Llama
Mam	Mammals
Mo	Mouse
Pl	Plant
Po	Porcine
Pr	Primate
Pro	Prokaryotes
Rb	Rabbit
Rt	Rat
Sh	Sheep
Sq	Squirrel
Vert	Vertebrates
Vi	Virus
Xe	Xenopus
Yt	Yeast
Zf	Zebrafish

AP	Affinity purification
Bl	Blocking
ChIP	Chromatin Immunoprecipitation
DB	Dot Blot
EMSA	Electron Microscopy
ELISA	Enzyme Linked Immunosorbent Assay
FACS	Flow Cytometry
FA	Functional Assay
GS	Gel Shift
ICC	Immunocytochemistry
ID	Immunodiffusion
IF	Immunofluorescence
IHC-F	Immunohistochemistry frozen
IHC-P	Immunohistochemistry paraffin
IP	Immunoprecipitation
IVT	In vivo targeting
Neu	Neutralising
Pep-ELISA	Peptide ELISA
PLA	Proximity Ligation Assay
RIA	Radioimmunoassay
WB	Western Blot

0 matching AKT1 Substrate 1 antibodies for Flow Cytometry from 16 suppliers

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0 matching AKT1 Substrate 1 antibodies for Flow Cytometry after applying filters

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Western Blot

Immunohistochemistry paraffin

Immunofluorescence

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